Review



calcein am pi double staining kit  (Dojindo Labs)


Bioz Verified Symbol Dojindo Labs is a verified supplier
Bioz Manufacturer Symbol Dojindo Labs manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 97

    Structured Review

    Dojindo Labs calcein am pi double staining kit
    Calcein Am Pi Double Staining Kit, supplied by Dojindo Labs, used in various techniques. Bioz Stars score: 97/100, based on 976 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/calcein+am+pi+kit/Calcein/pmc13081185-70-6-13
    Average 97 stars, based on 976 article reviews
    calcein am pi double staining kit - by Bioz Stars, 2026-10
    97/100 stars

    Images

    Related Articles

    Staining:

    Article Title: Discovery of Zidovudine as a cardiomyocyte protectant for doxorubicin-induced toxicity through high-throughput phenotypic drug screening
    Article Snippet: The drugs were individually added using the Tecan Freedom EVO 150 liquid Handler (Tecan). .. Cells were then treated for three days and subsequently stained using a Calcein-AM/PI kit (C542, DOJINDO) before being loaded onto the Operetta CLS High-Content Analysis System (PerkinElmer) for image capture. .. The number of live cells (Calcein-AM + /PI − ) and necrotic cells (PI + ) was quantified utilizing the Harmony 4.9 software (PerkinElmer).

    Article Title: Oxidized Albumin Induces Renal Tubular Cell Death and Promotes the Progression of Renal Diseases Through Ferroptosis
    Article Snippet: .. Live/dead staining was performed using a Calcein-AM/PI kit (Dojindo, Kumamoto, Japan) according to the manufacturer’s instructions. ..

    Article Title: Stromal homeostasis-restoring “rocket-like” nanomedicine inhibited pancreatic tumor growth in vivo
    Article Snippet: .. Triethylamine (TEA, Sigma-Aldrich); Cetyltrimethylammonium bromide (CTAB, Xinyanbomei); Sodium salicylate (NaSal, Sigma-Aldrich); Tetraethyl orthosilicate (TEOS, CATO); 1,2-Bis(triethoxysilyl)ethane (BTES, Xinhengyan); Ethanol; Hydrochloric acid; Methanol; Gemcitabine (MedChemExpress); Ammonium bicarbonate (Coolaber); Urokinase-type plasminogen activator (Solarbio); CCK-8 kit (CWBIO); ROS staining kit (Poolyue); Calcein-AM/PI kit (DOJINDO); anti-uPA antibody (HUABIO); Calcium chloride (Supelco); Indocyanine green (zrbiorise); Dulbecco's modified Eagle medium (DMEM, Sigma-Aldrich); Panc-1, Pan02, and L929 cells (ATCC); Fluorescein isothiocyanate (FITC, Qisong); 4′,6-diamidino-2-phenylindole (DAPI, Solarbio); DUTP (Roche); IPR-803 (MCE). ..

    Article Title: Oxidized Albumin Induces Renal Tubular Cell Death and Promotes the Progression of Renal Diseases Through Ferroptosis
    Article Snippet: .. Live/dead staining was performed using a Calcein-AM/PI kit (Dojindo, Kumamoto, Japan) according to the manufacturer’s instructions. ..

    High Content Screening:

    Article Title: Discovery of Zidovudine as a cardiomyocyte protectant for doxorubicin-induced toxicity through high-throughput phenotypic drug screening
    Article Snippet: The drugs were individually added using the Tecan Freedom EVO 150 liquid Handler (Tecan). .. Cells were then treated for three days and subsequently stained using a Calcein-AM/PI kit (C542, DOJINDO) before being loaded onto the Operetta CLS High-Content Analysis System (PerkinElmer) for image capture. .. The number of live cells (Calcein-AM + /PI − ) and necrotic cells (PI + ) was quantified utilizing the Harmony 4.9 software (PerkinElmer).

    CCK-8 Assay:

    Article Title: A metal-organic framework-based co-delivery system for atherosclerosis therapy via macrophage regulation.
    Article Snippet: Atherosclerosis is an inflammatory disease characterized by lipid deposition, inflammatory cell infiltration, and plaque formation, representing a primary underlying cause of cardiovascular diseases.. The complex pathogenesis of atherosclerosis limits the effectiveness of current therapies, which often demonstrate suboptimal efficacy.. In this study, we developed a nanocarrier-based delivery system combining fragile histidine triad protein (FHIT) with the copper-tetrakis(4-carboxyphenyl)porphyrin metal–organic framework (Cu-TCPP MOF) for atherosclerosis treatment.

    Article Title: Stromal homeostasis-restoring “rocket-like” nanomedicine inhibited pancreatic tumor growth in vivo
    Article Snippet: .. Triethylamine (TEA, Sigma-Aldrich); Cetyltrimethylammonium bromide (CTAB, Xinyanbomei); Sodium salicylate (NaSal, Sigma-Aldrich); Tetraethyl orthosilicate (TEOS, CATO); 1,2-Bis(triethoxysilyl)ethane (BTES, Xinhengyan); Ethanol; Hydrochloric acid; Methanol; Gemcitabine (MedChemExpress); Ammonium bicarbonate (Coolaber); Urokinase-type plasminogen activator (Solarbio); CCK-8 kit (CWBIO); ROS staining kit (Poolyue); Calcein-AM/PI kit (DOJINDO); anti-uPA antibody (HUABIO); Calcium chloride (Supelco); Indocyanine green (zrbiorise); Dulbecco's modified Eagle medium (DMEM, Sigma-Aldrich); Panc-1, Pan02, and L929 cells (ATCC); Fluorescein isothiocyanate (FITC, Qisong); 4′,6-diamidino-2-phenylindole (DAPI, Solarbio); DUTP (Roche); IPR-803 (MCE). ..

    Viability Assay:

    Article Title: Bioactive Magnesium Silicate Activating Myocardial Energy Metabolism For Infarcted Myocardium Repair
    Article Snippet: .. .9 Cell Viability Assay ell viability in 3D bioprinted cardiac patches was assessed by he live/dead assay using the Calcein-AM/PI kit (Dojindo, Japan). ..

    Modification:

    Article Title: Stromal homeostasis-restoring “rocket-like” nanomedicine inhibited pancreatic tumor growth in vivo
    Article Snippet: .. Triethylamine (TEA, Sigma-Aldrich); Cetyltrimethylammonium bromide (CTAB, Xinyanbomei); Sodium salicylate (NaSal, Sigma-Aldrich); Tetraethyl orthosilicate (TEOS, CATO); 1,2-Bis(triethoxysilyl)ethane (BTES, Xinhengyan); Ethanol; Hydrochloric acid; Methanol; Gemcitabine (MedChemExpress); Ammonium bicarbonate (Coolaber); Urokinase-type plasminogen activator (Solarbio); CCK-8 kit (CWBIO); ROS staining kit (Poolyue); Calcein-AM/PI kit (DOJINDO); anti-uPA antibody (HUABIO); Calcium chloride (Supelco); Indocyanine green (zrbiorise); Dulbecco's modified Eagle medium (DMEM, Sigma-Aldrich); Panc-1, Pan02, and L929 cells (ATCC); Fluorescein isothiocyanate (FITC, Qisong); 4′,6-diamidino-2-phenylindole (DAPI, Solarbio); DUTP (Roche); IPR-803 (MCE). ..



    Similar Products

    97
    Dojindo Labs calcein am pi double staining kit
    Calcein Am Pi Double Staining Kit, supplied by Dojindo Labs, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/calcein+am+pi+kit/Calcein/pmc13081185-70-6-13
    Average 97 stars, based on 1 article reviews
    calcein am pi double staining kit - by Bioz Stars, 2026-10
    97/100 stars
      Buy from Supplier

    86
    Yeasen Biotechnology calcein am pi double stain kit
    Calcein Am Pi Double Stain Kit, supplied by Yeasen Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/calcein+am+pi+kit/annexin+apoptosis+detection+fitc+kit+pi+v/pmc13261646-328-1-8
    Average 86 stars, based on 1 article reviews
    calcein am pi double stain kit - by Bioz Stars, 2026-10
    86/100 stars
      Buy from Supplier

    86
    Servicebio Inc calcein am pi double staining kit
    Calcein Am Pi Double Staining Kit, supplied by Servicebio Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/calcein+am+pi+kit/am+calcein/pmc13273694-47-7-11
    Average 86 stars, based on 1 article reviews
    calcein am pi double staining kit - by Bioz Stars, 2026-10
    86/100 stars
      Buy from Supplier

    97
    Guangzhou JET Bio-Filtration e-click edu cell proliferation imaging assay kit
    E Click Edu Cell Proliferation Imaging Assay Kit, supplied by Guangzhou JET Bio-Filtration, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/calcein+am+pi+kit/E-Click+EdU+Cell+Proliferation+Imaging+Assay+Kit/custom%40e-ck-a376%4042542056
    Average 97 stars, based on 1 article reviews
    e-click edu cell proliferation imaging assay kit - by Bioz Stars, 2026-10
    97/100 stars
      Buy from Supplier

    99
    Beyotime calcein am pi double staining kit
    Calcein Am Pi Double Staining Kit, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/calcein+am+pi+kit/Calcein+AM/pmc12926989-407-9-12
    Average 99 stars, based on 1 article reviews
    calcein am pi double staining kit - by Bioz Stars, 2026-10
    99/100 stars
      Buy from Supplier

    86
    Abbkine Inc calcein am propidium iodide pi kit
    Calcein Am Propidium Iodide Pi Kit, supplied by Abbkine Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/calcein+am+pi+kit/iodide+propidium/pmc13247127-97-6-11
    Average 86 stars, based on 1 article reviews
    calcein am propidium iodide pi kit - by Bioz Stars, 2026-10
    86/100 stars
      Buy from Supplier

    86
    Keygen Biotech calcein am pi cell viability cytotoxicity assay kit
    Calcein Am Pi Cell Viability Cytotoxicity Assay Kit, supplied by Keygen Biotech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/calcein+am+pi+kit/am+calcein/pmc13067122-189-1-16
    Average 86 stars, based on 1 article reviews
    calcein am pi cell viability cytotoxicity assay kit - by Bioz Stars, 2026-10
    86/100 stars
      Buy from Supplier

    97
    Beyotime calcein am pi cell viability cytotoxicity kit
    In vitro antifibrotic effects of sEVs ErF on HSFs. (A, B) EdU staining and quantification of proliferating HSFs treated with Con, Er, sEVs Er , or sEVs ErF . Scale bar, 100 μm. (C) CCK-8 assay showing dose-dependent viability changes. (D, E) Transwell migration images and quantification of migrated HSFs. Scale bar, 100 μm. (F, G) Wound healing assay images and migration area (%) over time. Scale bar, 400 μm. (H-K) Western blot and densitometric analysis of COL I, COL III, and α-SMA; GAPDH, loading control. (L, M) α-SMA immunofluorescence and quantitative fluorescence intensity in HSFs. Scale bar, 50 μm. <t>(N,</t> <t>O)</t> <t>Calcein-AM/PI</t> flow cytometry and quantification of PI-positive cells. Data are mean ± SD; ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001.
    Calcein Am Pi Cell Viability Cytotoxicity Kit, supplied by Beyotime, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/calcein+am+pi+kit/Calcein%2FPI+Cell+Viability%2FCytotoxicity+Assay+Kit/pmc13098611-134-8-12
    Average 97 stars, based on 1 article reviews
    calcein am pi cell viability cytotoxicity kit - by Bioz Stars, 2026-10
    97/100 stars
      Buy from Supplier

    97
    Dojindo Labs calcein am pi kit
    In vitro antifibrotic effects of sEVs ErF on HSFs. (A, B) EdU staining and quantification of proliferating HSFs treated with Con, Er, sEVs Er , or sEVs ErF . Scale bar, 100 μm. (C) CCK-8 assay showing dose-dependent viability changes. (D, E) Transwell migration images and quantification of migrated HSFs. Scale bar, 100 μm. (F, G) Wound healing assay images and migration area (%) over time. Scale bar, 400 μm. (H-K) Western blot and densitometric analysis of COL I, COL III, and α-SMA; GAPDH, loading control. (L, M) α-SMA immunofluorescence and quantitative fluorescence intensity in HSFs. Scale bar, 50 μm. <t>(N,</t> <t>O)</t> <t>Calcein-AM/PI</t> flow cytometry and quantification of PI-positive cells. Data are mean ± SD; ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001.
    Calcein Am Pi Kit, supplied by Dojindo Labs, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/calcein+am+pi+kit/Calcein/pmc13091541-179-38-40
    Average 97 stars, based on 1 article reviews
    calcein am pi kit - by Bioz Stars, 2026-10
    97/100 stars
      Buy from Supplier

    Image Search Results


    In vitro antifibrotic effects of sEVs ErF on HSFs. (A, B) EdU staining and quantification of proliferating HSFs treated with Con, Er, sEVs Er , or sEVs ErF . Scale bar, 100 μm. (C) CCK-8 assay showing dose-dependent viability changes. (D, E) Transwell migration images and quantification of migrated HSFs. Scale bar, 100 μm. (F, G) Wound healing assay images and migration area (%) over time. Scale bar, 400 μm. (H-K) Western blot and densitometric analysis of COL I, COL III, and α-SMA; GAPDH, loading control. (L, M) α-SMA immunofluorescence and quantitative fluorescence intensity in HSFs. Scale bar, 50 μm. (N, O) Calcein-AM/PI flow cytometry and quantification of PI-positive cells. Data are mean ± SD; ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001.

    Journal: Materials Today Bio

    Article Title: Rational design of FAP-targeted sEVs delivered by microneedles for precision treatment of hypertrophic scars via ferroptosis in hypertrophic scar fibroblasts

    doi: 10.1016/j.mtbio.2026.103117

    Figure Lengend Snippet: In vitro antifibrotic effects of sEVs ErF on HSFs. (A, B) EdU staining and quantification of proliferating HSFs treated with Con, Er, sEVs Er , or sEVs ErF . Scale bar, 100 μm. (C) CCK-8 assay showing dose-dependent viability changes. (D, E) Transwell migration images and quantification of migrated HSFs. Scale bar, 100 μm. (F, G) Wound healing assay images and migration area (%) over time. Scale bar, 400 μm. (H-K) Western blot and densitometric analysis of COL I, COL III, and α-SMA; GAPDH, loading control. (L, M) α-SMA immunofluorescence and quantitative fluorescence intensity in HSFs. Scale bar, 50 μm. (N, O) Calcein-AM/PI flow cytometry and quantification of PI-positive cells. Data are mean ± SD; ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001.

    Article Snippet: Cell viability and death were assessed using a Calcein-AM/PI Cell Viability/Cytotoxicity Kit (Beyotime, China).

    Techniques: In Vitro, Staining, CCK-8 Assay, Migration, Wound Healing Assay, Western Blot, Control, Immunofluorescence, Fluorescence, Flow Cytometry